| 产品编号 | bs-3792R |
| 英文名称 | CR2 Rabbit pAb |
| 中文名称 | 2型补体受体（CD21）抗体 |
| 别 名 | C3DR; CD 21; CD21; CD21 antigen; Complement C3d receptor; Complement component(3d/Epstein Barr virus) receptor 2; Complement component receptor 2; Complement receptor type 2; Cr 2; EBV receptor; Epstein Barr virus receptor; CR2_HUMAN. |
| 研究领域 | 细胞生物 免疫学 细胞膜受体 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 111 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CR2: 981-1092/1092 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] Function: Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. Subunit: Interacts (via Sushi domain 1 and 2) with C3dg. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen. DISEASE: Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Defects in CR2 are the cause of immunodeficiency, common variable, type 7 (CVID7) [MIM:614699]. A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. Similarity: Belongs to the receptors of complement activation (RCA) family. Contains 15 Sushi (CCP/SCR) domains. SWISS: P20023 Gene ID: 1380 Database links: Entrez Gene: 1380 Human Entrez Gene: 12902 Mouse Omim: 120650 Human SwissProt: P20023 Human SwissProt: P19070 Mouse Unigene: 445757 Human Unigene: 235387 Mouse |
| 产品图片 |
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD21 (bs-3792R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
Blank control(blue): U-87MG Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (bs-3792R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (bs-3792R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Blank control: Raji cells(blue). Primary Antibody:Rabbit Anti- CD21 antibody(bs-3792R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
